| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 1.0 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IGFBP-2 at 5 Mu g/mL (100 Mu L/well) can bind Recombinant Human IGF1 with a linear range of 53-212 ng/mL. |
| Gene Symbol: |
IGFBP2 |
| Gene ID: |
3485 |
| Uniprot ID: |
IBP2_HUMAN |
| Immunogen Region: |
Glu40-Gln328 |
| Immunogen: |
Recombinant Human IGFBP-2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu40-Gln328) of human IGFBP-2 (Accession #NP_000588.2) fused with an initial Met at the N-terminus and a 6×His tag at the |
| Immunogen Sequence: |
EVLFRCPPCTPERLAACGPP PVAPPAAVAAVAGGARMPCA ELVREPGCGCCSVCARLEGE ACGVYTPRCGQGLRCYPHPG SELPLQALVMGEGTCEKRRD AEYGASPEQVADNGDDHSEG GLVENHVDSTMNMLGGGGSA GRKPLKSGMKELAVFREKVT EQHRQMGKGGKHHLGLEEPK KLRPPPARTPCQQELDQVLE RISTMRLPDERGPLEHLYSL HIPNCDKHGLYNLKQCKMS |
| Post Translational Modifications | Cleaved by MMP9 leading to release of free IGF2 from IGFBP2-IGF2 complex, which contributes to enhance the motility and the growth of astrocytes. O-glycosylated. |
| Function | Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Functions coordinately with receptor protein tyrosine phosphatase beta/PTPRB and the IGF1 receptor to regulate IGF1-mediated signaling by stimulating the phosphorylation of PTEN leading to its inactivation and AKT1 activation. Plays a positive role in cell migration via interaction with integrin alpha5/ITGA5 through an RGD motif. Additionally, interaction with ITGA5/ITGB1 enhances the adhesion of endothelial progenitor cells to endothelial cells. Upon mitochondrial damage, facilitates apoptosis with ITGA5 of podocytes, and then activates the phosphorylation of focal adhesion kinase (FAK)-mediated mitochondrial injury. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 2Ibp-2Igf-Binding Protein 2Igfbp-2 |
| Database Links | Reactome: R-HSA-381426 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Insulin-Like Growth Factor-Binding Protein 2 proteinIbp-2 proteinIgf-Binding Protein 2 proteinIgfbp-2 proteinIGFBP2 proteinBP2 proteinIBP2 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
