| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IGFBP-1 at 5 Mu g/mL (100 Mu L/well) can bind Recombinant Human IGF1 with a linear range of 44-176 ng/mL. |
| Gene Symbol: |
IGFBP1 |
| Gene ID: |
3484 |
| Uniprot ID: |
IBP1_HUMAN |
| Immunogen Region: |
Ala26-Asn259 |
| Immunogen: |
Recombinant Human IGFBP-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala26-Asn259) of human IGFBP-1 (Accession #NP_000587.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: |
APWQCAPCSAEKLALCPPVS ASCSEVTRSAGCGCCPMCAL PLGAACGVATARCARGLSCR ALPGEQQPLHALTRGQGACV QESDASAPHAAEAGSPESPE STEITEEELLDNFHLMAPSE EDHSILWDAISTYDGSKALH VTNIKKWKEPCRIELYRVVE SLAKAQETSGEEISKFYLPN CNKNGFYHSRQCETSMDGEA GLCWCVYPWNGKRIPGSPEI RGDPNCQIYFNVQN |
| Post Translational Modifications | Phosphorylated.probably by casein kinase II. Phosphorylation alters the affinity of the protein for IGFs. In amniotic fluid, the unmodified protein is the most abundant form, while mono-, bi-, tri- and tetraphosphorylated forms are present in decreasing amounts. The phosphorylation state may influence the propensity to proteolysis. |
| Function | Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including cell migration, proliferation, differentiation or apoptosis in a cell-type specific manner. Plays also a positive role in cell migration by interacting with integrin ITGA5:ITGB1 through its RGD motif. Mechanistically, binding to integrins leads to activation of focal adhesion kinase/PTK2 and stimulation of the mitogen-activated protein kinase (MAPK) pathway. Regulates cardiomyocyte apoptosis by suppressing HIF-1alpha/HIF1A ubiquitination and subsequent degradation. |
| Protein Name | Insulin-Like Growth Factor-Binding Protein 1Ibp-1Igf-Binding Protein 1Igfbp-1Placental Protein 12Pp12 |
| Database Links | Reactome: R-HSA-380994Reactome: R-HSA-381426Reactome: R-HSA-8957275Reactome: R-HSA-9615017 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Insulin-Like Growth Factor-Binding Protein 1 proteinIbp-1 proteinIgf-Binding Protein 1 proteinIgfbp-1 proteinPlacental Protein 12 proteinPp12 proteinIGFBP1 proteinIBP1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
