| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human IGF-1R (CD221)-protein was developed from hek293. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | 0.2 Mu m filtered PBS solution, pH7.2. |
| Dilution Range: | A quick spin of the vial followed by gently mixing in distilled water to prepare a stock solution concentration not less than 0.1 mg/mL. Avoid vigorous shaking or vortex |
| Storage Instruction: | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year, addition of a carrier protein to your working aliquots (0.1% BSA or HSA) is recommended for long term storage Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The activity of recombinant IGF-1R was measured by its ability to bind Human IGF-1 in a functional ELISA assay. NA |
| Immunogen Region: | ECD |
| Immunogen: | Optimized DNA sequence encoding extracellular domain of human IGF-1R (CD221) including a C-terminal 6His tag was expressed in HEK293 cells. NA |
| Background | Recombinant human IGF-1R (CD221) is a heterodimer protein, due to glycosylation migrates as an approximately 50kDa, 120kDa, 150kDa protein under reducing ocnditions on SDS-PAGE. |
Information sourced from Uniprot.org