This HTT Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids..
Applications
ELISA
Reactivity
Human
Sensitivity
0.064ng/mL
Detection Limit
0.156-10ng/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
tissue homogenates, cell lysates or other biological fluids.
Additional Info
Tissue Specificity
Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
Post Translational Modifications
Huntingtin: Cleaved by caspases downstream of the polyglutamine stretch. The resulting N-terminal fragments are cytotoxic and provokes apoptosis. Huntingtin: Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation. Huntingtin: Phosphorylation at Ser-1179 and Ser-1199 by CDK5 in response to DNA damage in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. Huntingtin, myristoylated N-terminal fragment: Myristoylated at Gly-551, following proteolytic cleavage at Asp-550.
Function
Huntingtin: May play a role in microtubule-mediated transport or vesicle function. Huntingtin, myristoylated N-terminal fragment: Promotes the formation of autophagic vesicles.
Protein Name
HuntingtinHuntington Disease ProteinHd Protein Cleaved Into - Huntingtin - Myristoylated N-Terminal Fragment
Huntingtin: CytoplasmNucleusEarly EndosomeThe Mutant Huntingtin Protein Colocalizes With Akap8l In The Nuclear Matrix Of Huntington Disease NeuronsShuttles Between Cytoplasm And Nucleus In A Ran Gtpase-Independent MannerRecruits Onto Early Endosomes In A Rab5- And Hap40-Dependent FashionHuntingtinMyristoylated N-Terminal Fragment: Cytoplasmic VesicleAutophagosome
Alternative ELISA Names
Huntingtin ELISA kitHuntington Disease Protein ELISA kitHd Protein Cleaved Into - Huntingtin - Myristoylated N-Terminal Fragment ELISA kitHTT ELISA kitHD ELISA kitIT15 ELISA kit
