| Host: |
Rabbit/Rabbit |
| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Short Description: |
This HSP-27/HSPB1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Conjugation: |
Unconjugated/Biotin |
| Formulation: |
Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Purification: |
Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Concentration: |
0.5 mg/mL |
| Storage Instruction: |
Store at-20°C for long term, and avoid freeze-thaw cycles. |
| Determination Method: |
Colormetric |
| Gene Symbol: |
HSPB1 |
| Gene ID: |
3315 |
| Uniprot ID: |
HSPB1_HUMAN |
| Immunogen: |
Capture immunogen: Recombinant Human HSP-27/HSPB1 protien.Detection Immunogen: Recombinant Human HSP-27/HSPB1 protien. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated upon exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress dissociates HSPB1 from large small heat-shock protein (sHsps) oligomers and impairs its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement. |
| Function | Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. |
| Protein Name | Heat Shock Protein Beta-1Hspb128 Kda Heat Shock ProteinEstrogen-Regulated 24 Kda ProteinHeat Shock 27 Kda ProteinHsp 27Heat Shock Protein Family B Member 1Stress-Responsive Protein 27Srp27 |
| Database Links | Reactome: R-HSA-4420097Reactome: R-HSA-450408Reactome: R-HSA-5687128Reactome: R-HSA-9009391 |
| Cellular Localisation | CytoplasmNucleusCytoskeletonSpindleCytoplasmic In Interphase CellsColocalizes With Mitotic Spindles In Mitotic CellsTranslocates To The Nucleus During Heat Shock And Resides In Sub-Nuclear Structures Known As Sc35 Speckles Or Nuclear Splicing Speckles |
| Alternative Antibody Pair Names | Heat Shock Protein Beta-1 antibody pairHspb1 antibody pair28 Kda Heat Shock Protein antibody pairEstrogen-Regulated 24 Kda Protein antibody pairHeat Shock 27 Kda Protein antibody pairHsp 27 antibody pairHeat Shock Protein Family B Member 1 antibody pairStress-Responsive Protein 27 antibody pairSrp27 antibody pairHSPB1 antibody pairHSP27 antibody pairHSP28 antibody pair |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
