Human HSD3b1 (3-Beta-Hydroxysteroid Dehydrogenase Type 1) Sandwich ELISA Kit (STJE0009533)
SPECIFICATIONS
General Information
| Short Description | This HSD3b1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.114ng/mL |
| Detection Limit | 0.312-20ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | HSD3B1 |
| Gene ID | 3283 |
| Uniprot ID | 3BHS1_HUMAN |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Additional Info
| Tissue Specificity | Placenta and skin. Predominantly expressed in mammary gland tissue. |
| Post Translational Modifications | |
| Function | A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD(+) as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable). |
| Protein Name | 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 13 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I3-Beta-Hsd I3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase3-Beta-Hydroxysteroid 3-DehydrogenaseDelta-5-3-Ketosteroid IsomeraseDihydrotestosterone OxidoreductaseSteroid Delta-IsomeraseTrophoblast Antigen Fdo161g |
| Database Links | Reactome: R-HSA-193048Reactome: R-HSA-193993Reactome: R-HSA-194002 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinMitochondrion Membrane |
| Alternative ELISA Names | 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1 ELISA kit3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I ELISA kit3-Beta-Hsd I ELISA kit3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase ELISA kit3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase ELISA kit3-Beta-Hydroxysteroid 3-Dehydrogenase ELISA kitDelta-5-3-Ketosteroid Isomerase ELISA kitDihydrotestosterone Oxidoreductase ELISA kitSteroid Delta-Isomerase ELISA kitTrophoblast Antigen Fdo161g ELISA kitHSD3B1 ELISA kit3BH ELISA kitHSDB3A ELISA kit |
| output |
Information sourced from Uniprot.org