Human Hexokinase 2 protein (Recombinant) (His-Tag) (STJP019671)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Hexokinase 2-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Specific activity is > 12, 000pmol/min/ug. One unit will convert 1pmole of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37C. |
Target Information
| Gene Symbol | HK2 |
| Gene ID | 3099 |
| Uniprot ID | HXK2_HUMAN |
| Accession Number | NP_000180 |
| Immunogen | Human |
| Immunogen Region | 1-917aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. |
| Function | Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis. |
| Protein Name | Hexokinase-2Hexokinase Type IiHk IiHexokinase-BMuscle Form Hexokinase |
| Database Links | Reactome: R-HSA-70171 |
| Cellular Localisation | Mitochondrion Outer MembranePeripheral Membrane ProteinCytoplasmCytosolThe Mitochondrial-Binding Peptide (Mbp) Region Promotes Association With The Mitochondrial Outer MembraneThe Interaction With The Mitochondrial Outer Membrane Via The Mitochondrial-Binding Peptide (Mbp) Region Promotes Higher Stability Of The ProteinRelease From The Mitochondrial Outer Membrane Into The Cytosol Induces Permeability Transition Pore (Ptp) Opening And Apoptosis |
| Alternative Protein Names | Hexokinase-2 proteinHexokinase Type Ii proteinHk Ii proteinHexokinase-B proteinMuscle Form Hexokinase proteinHK2 protein |
Information sourced from Uniprot.org