Human GTF2B protein (Recombinant) (N-GST) (STJP014593)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human GTF2B-N-GST protein was developed from e.coli and has a target region of N-GST. For use in research applications. |
| Applications | ELISA/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a 0.22 Mu m filtered solution in 20mM Tris-HCl, 100mM NaCl, pH 8.0. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8ยฐC for one week. Store at-20 to-80ยฐC for twelve months from the date of receipt. |
| Endotoxin | < 1 EU/ยตg as determined by LAL test. |
Target Information
| Gene Symbol | GTF2B |
| Gene ID | 2959 |
| Uniprot ID | TF2B_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Leu316 |
Additional Info
| Post Translational Modifications | Acetylated. Autoacetylated.autoacetylation at Lys-238 stimulates transcription activation. |
| Function | General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner.these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-GCGCGACGCC-3', and downstream (BREd), 5'-GATTGATGGTTGTG-3', of the TATA box element. Modulates transcription start site selection. Also exhibits autoacetyltransferase activity that contributes to the activated transcription. |
| Protein Name | Transcription Initiation Factor IibGeneral Transcription Factor TfiibS300-Ii |
| Database Links | Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-674695Reactome: R-HSA-6807505Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-75953Reactome: R-HSA-76042 |
| Cellular Localisation | NucleusChromosomeNon-Acetylated Form Colocalizes With Dna In The G0/1S And G2 Phases Of The Cell CycleBut Not During MitosisAcetylated Form Colocalizes At Transcriptionally Silent Mitotic Chromatids During Mitosis At MetaphaseAnaphaseAnd Telophase Phases Of The Cell Cycle |
| Alternative Protein Names | Transcription Initiation Factor Iib proteinGeneral Transcription Factor Tfiib proteinS300-Ii proteinGTF2B proteinTF2B proteinTFIIB protein |
Information sourced from Uniprot.org