Human Growth hormone receptor/GHR protein (Recombinant) (C-hFc & His) (STJP002605)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Growth hormone receptor/GHR Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala 27-Tyr 264) of human Growth Hormone Receptor (GHR) (Accession #NP_000154.1) fused with an Fc, 6×His tag
General Information
| Short Description | Recombinant-Human Growth hormone receptor/GHR-C-hFc & His protein was developed in hek293 cells using the region Ala27-Tyr264. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Human GH at 2 Mu g/mL (100 Mu L/well) can bind Human GHR with a linear range of 0.1-8.58 ng/mL. |
| Determination Method | < 0.01 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | GHR |
| Gene ID | 2690 |
| Uniprot ID | GHR_HUMAN |
| Immunogen | Recombinant Human Growth hormone receptor/GHR Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala 27-Tyr 264) of human Growth Hormone Receptor (GHR) (Accession #NP_000154.1) fused with an Fc, 6×His tag |
| Immunogen Region | Ala27-Tyr264 |
| Immunogen Sequence | AILSRAPWSLQSVNPGLKTN SSKEPKFTKCRSPERETFSC HWTDEVHHGTKNLGPIQLFY TRRNTQEWTQEWKECPDYVS AGENSCYFNSSFTSIWIPYC IKLTSNGGTVDEKCFSVDEI VQPDPPIALNWTLLNVSLTG IHADIQVRWEAPRNADIQKG WMVLEYELQYKEVNETKWKM MDPILTTSVPVYSLKVDKEY EVRVRSKQRNSGNYGEFSEV LYVTLPQMSQFTCEEDFY |
Additional Info
| Post Translational Modifications | The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17. On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2. Ubiquitinated by the ECS(SOCS2) complex following ligand-binding and phosphorylation by JAK2, leading to its degradation by the proteasome. Regulation by the ECS(SOCS2) complex acts as a negative feedback loop of growth hormone receptor signaling. Ubiquitination is not sufficient for GHR internalization. |
| Function | Receptor for pituitary gland growth hormone (GH1) involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. Growth hormone-binding protein: The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. Isoform 2: Up-regulates the production of the soluble Growth hormone-binding protein form (GHBP) and acts as a negative inhibitor of growth hormone signaling. |
| Protein Name | Growth Hormone ReceptorGh ReceptorSomatotropin Receptor Cleaved Into - Growth Hormone-Binding ProteinGh-Binding ProteinGhbpSerum-Binding Protein |
| Database Links | Reactome: R-HSA-1170546Reactome: R-HSA-982772 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinOn Growth Hormone BindingGhr Is UbiquitinatedInternalizedDown-Regulated And Transported Into A Degradative Or Non-Degradative PathwayIsoform 2: Cell MembraneRemains Fixed To The Cell Membrane And Is Not InternalizedGrowth Hormone-Binding Protein: SecretedComplexed To A Substantial Fraction Of Circulating Gh |
| Alternative Protein Names | Growth Hormone Receptor proteinGh Receptor proteinSomatotropin Receptor Cleaved Into - Growth Hormone-Binding Protein proteinGh-Binding Protein proteinGhbp proteinSerum-Binding Protein proteinGHR protein |
Information sourced from Uniprot.org