| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.115ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description: | This GRIA2 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
| Gene Symbol: | GRIA2 |
| Gene ID: | 2891 |
| Uniprot ID: | GRIA2_HUMAN |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Palmitoylated. Depalmitoylated upon L-glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. Ubiquitinated by RNF167, leading to its degradation. Phosphorylation at Tyr-876 is required for interaction with IQSEC1 and ARF6 activation, which in turn triggers AMPAR internalization for persistent synaptic depression. N-glycosylated. |
| Function | Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate. Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting. |
| Protein Name | Glutamate Receptor 2 Glur-2 Ampa-Selective Glutamate Receptor 2 Glur-B Glur-K2 Glutamate Receptor Ionotropic - Ampa 2 |
| Database Links | Reactome: R-HSA-399710 Reactome: R-HSA-416993 Reactome: R-HSA-438066 Reactome: R-HSA-9022699 Reactome: R-HSA-9620244 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Postsynaptic Cell Membrane Postsynaptic Density Membrane Interaction With Cacng2 Cnih2 And Cnih3 Promotes Cell Surface Expression Displays A Somatodendritic Localization And Is Excluded From Axons In Neurons |
| Alternative ELISA Names | Glutamate Receptor 2 ELISA kit Glur-2 ELISA kit Ampa-Selective Glutamate Receptor 2 ELISA kit Glur-B ELISA kit Glur-K2 ELISA kit Glutamate Receptor Ionotropic - Ampa 2 ELISA kit GRIA2 ELISA kit GluA2 ELISA kit GLUR2 ELISA kit |
| output |
Information sourced from Uniprot.org
