Human GNAI3 protein (Recombinant) (His-Tag) (STJP017605)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human GNAI3-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% Glycerol, 100mM NaCl |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GNAI3 |
| Gene ID | 2773 |
| Uniprot ID | GNAI3_HUMAN |
| Accession Number | NP_006487 |
| Immunogen | Human |
| Immunogen Region | 1-354aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MGSMGCTLSA EDKAAVERSK MIDRNLREDG EKAAKEVKLL LLGAGESGKS TIVKQMKIIH EDGYSEDECK QYKVVVYSNT IQSIIAIIRA MGRLKIDFGE AARADDARQL FVLAGSAEEG VMTPELAGVI KRLWRDGGVQ ACFSRSREYQ LNDSASYYLN DLDRISQSNY IPTQQDVLRT RVKTTGIVET HFTFKDLYFK MFDVGGQR |
Additional Info
| Post Translational Modifications | (Microbial infection) Deamidated at Gln-204 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA. |
| Function | Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels. Stimulates the activity of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. The active GTP-bound form activates the calcium permeant TRPC5 ion channels. |
| Protein Name | Guanine Nucleotide-Binding Protein G(I Subunit Alpha-3G(I Alpha-3 |
| Database Links | Reactome: R-HSA-170670Reactome: R-HSA-392170Reactome: R-HSA-418555Reactome: R-HSA-418594Reactome: R-HSA-418597Reactome: R-HSA-9009391Reactome: R-HSA-9634597Reactome: R-HSA-9660821 |
| Cellular Localisation | CytoplasmCell MembraneLipid-AnchorCytoskeletonMicrotubule Organizing CenterCentrosomeLocalizes In The Centrosomes Of Interphase And Mitotic CellsDetected At The Cleavage Furrow And/Or The Midbody |
| Alternative Protein Names | Guanine Nucleotide-Binding Protein G(I Subunit Alpha-3 proteinG(I Alpha-3 proteinGNAI3 protein |
Information sourced from Uniprot.org