Human Glypican 1/GPC1 (Glypican 1/GPC1) Antibody Pair (STJAP00871)
General Information
| Short Description | This Glypican 1/GPC1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R07-6H-3/R07-8H-3 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | GPC1 |
| Gene ID | 2817 |
| Uniprot ID | GPC1_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Post Translational Modifications | S-nitrosylated in a Cu(2+)-dependent manner. Nitric acid (NO) is released from the nitrosylated cysteines by ascorbate or by some other reducing agent, in a Cu(2+) or Zn(2+) dependent manner. This free nitric oxide is then capable of cleaving the heparan sulfate side chains. N- and O-glycosylated. N-glycosylation is mainly of the complex type containing sialic acid. O-glycosylated with heparan sulfate. The heparan sulfate chains can be cleaved either by the action of heparanase or, degraded by a deaminative process that uses nitric oxide (NO) released from the S-nitrosylated cysteines. This process is triggered by ascorbate, or by some other reducing agent, in a Cu(2+)- or Zn(2+) dependent manner. Cu(2+) ions are provided by ceruloproteins such as APP, PRNP or CP which associate with GCP1 in intracellular compartments or lipid rafts. This cell-associated glypican is further processed to give rise to a medium-released species. |
| Function | Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. |
| Protein Name | Glypican-1 Cleaved Into - Secreted Glypican-1 |
| Database Links | Reactome: R-HSA-1971475Reactome: R-HSA-2022928Reactome: R-HSA-2024096Reactome: R-HSA-202733Reactome: R-HSA-3560783Reactome: R-HSA-3560801Reactome: R-HSA-3656237Reactome: R-HSA-3656253Reactome: R-HSA-376176Reactome: R-HSA-4420332Reactome: R-HSA-9694614Reactome: R-HSA-975634Reactome: R-HSA-9820960Reactome: R-HSA-9833110 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorExtracellular SideEndosomeS-Nitrosylated Form Recycled In EndosomesLocalizes To Cav1-Containing Vesicles Close To The Cell SurfaceCleavage Of Heparan Sulfate Side Chains Takes Place Mainly In Late EndosomesAssociates With Both Forms Of Prnp In Lipid RaftsColocalizes With App In Perinuclear Compartments And With Cp In Intracellular CompartmentsAssociates With Fibrillar App Amyloid-Beta Peptides In Lipid Rafts In Alzheimer Disease BrainsSecreted Glypican-1: SecretedExtracellular Space |
| Alternative Antibody Pair Names | Glypican-1 Cleaved Into - Secreted Glypican-1 antibody pairGPC1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Glypican 1/GPC1 (Glypican 1/GPC1) Antibody Pair (STJAP00871)
This antibody pair is designed to detect human Glypican 1 (GPC1), corresponding to UniProt entry P35052. Reactivity has been confirmed for human samples only.
This antibody pair has been validated for use in ELISA. It is intended for sandwich ELISA development using one antibody as the capture and the other as the detection antibody.
Both antibodies are rabbit monoclonal IgGs (clone IDs R07-6H-3 and R07-8H-3) supplied unconjugated in 0.01M PBS at pH 7.2, and purified by affinity chromatography. Neither antibody carries a conjugate or tag, so end users are responsible for labeling the detection antibody as required.
Optimal working concentrations are not predefined for this product and must be determined by the end user through titration during assay development.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and keep at -20°C, and avoid repeated freeze/thaw cycles to maintain activity.
Both antibodies were raised against a recombinant human GPC1 protein. No specific immunogen region is indicated in the product documentation.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.