Human Glutathione S-transferase alpha 1/GSTA1 protein (Recombinant) (No-Tag) (STJP016585)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Glutathione S-transferase alpha 1/GSTA1-No-Tag protein was developed from e.coli and has a target region of No-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity | Specific activity is > 35, 000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2, 4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C. |
Target Information
| Gene Symbol | GSTA1 |
| Gene ID | 2938 |
| Uniprot ID | GSTA1_HUMAN |
| Accession Number | NP_665683 |
| Immunogen | Human |
| Immunogen Region | 1-222aa |
| Immunogen Sequence | MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF |
Additional Info
| Tissue Specificity | Liver. |
| Function | Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis. Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid. |
| Protein Name | Glutathione S-Transferase A113-Hydroperoxyoctadecadienoate PeroxidaseAndrost-5-Ene-3 -17-Dione IsomeraseGst Ha Subunit 1Gst Class-Alpha Member 1Gst-EpsilonGsta1-1Gth1 Cleaved Into - Glutathione S-Transferase A1 - N-Terminally Processed |
| Database Links | Reactome: R-HSA-156590Reactome: R-HSA-189483Reactome: R-HSA-9748787Reactome: R-HSA-9818027 |
| Cellular Localisation | Cytoplasm |
| Alternative Protein Names | Glutathione S-Transferase A1 protein13-Hydroperoxyoctadecadienoate Peroxidase proteinAndrost-5-Ene-3 -17-Dione Isomerase proteinGst Ha Subunit 1 proteinGst Class-Alpha Member 1 proteinGst-Epsilon proteinGsta1-1 proteinGth1 Cleaved Into - Glutathione S-Transferase A1 - N-Terminally Processed proteinGSTA1 protein |
Information sourced from Uniprot.org