| Post Translational Modifications | Undergoes AFG3L2-mediated proteolytic degradation, upon hyperpolarization of mitochondria. |
| Function | Plays an important role in maintenance of mitochondrial morphology and in mediating either calcium or potassium/proton antiport. Mediates proton-dependent calcium efflux from mitochondrion. Functions also as an electroneutral mitochondrial proton/potassium exchanger. Required for the mitochondrial tubular network and cristae organization. Involved in apoptotic release of cytochrome c. Inhibits the proteolytic activity of AFG3L2, stimulating respiration and stabilizing respiratory enzymes in actively respiring mitochondria. However, when mitochondria become hyperpolarized, GHITM loses its inhibitory activity toward AFG3L2 and the now the active AFG3L2 turns first on GHITM and, if hyperpolarization persists, on other proteins of the mitochondria, leading to a broad remodeling of the mitochondrial proteome. |
| Protein Name | Growth Hormone-Inducible Transmembrane ProteinDermal Papilla-Derived Protein 2Mitochondrial Morphology And Cristae Structure 1Mics1Transmembrane Bax Inhibitor Motif-Containing Protein 5 |
| Cellular Localisation | Mitochondrion Inner MembraneMulti-Pass Membrane Protein |
| Alternative CLIA Names | Growth Hormone-Inducible Transmembrane Protein CLIA kitDermal Papilla-Derived Protein 2 CLIA kitMitochondrial Morphology And Cristae Structure 1 CLIA kitMics1 CLIA kitTransmembrane Bax Inhibitor Motif-Containing Protein 5 CLIA kitGHITM CLIA kitDERP2 CLIA kitMICS1 CLIA kitTMBIM5 CLIA kitMy021 CLIA kitUNQ244 CLIA kitPRO281 CLIA kit |
| Specificity | This kit recognizes Human GHITM in samples. No significant cross-reactivity or interference between Human GHITM and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-99 | 94 |
| EDTA plasma(n=8) | 94-108 | 101 |
| Cell culture media(n=8) | 100-115 | 107 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 194.09 | 360.48 | 1529.81 | 201.38 | 354.10 | 1499.46 |
| Standard deviation | 16.15 | 39.80 | 122.69 | 19.39 | 26.38 | 155.94 |
| CV (%) | 8.32 | 11.04 | 8.02 | 9.63 | 7.45 | 10.40 |
