Human GFRAL protein (Recombinant-Active) (C-His) (STJP012174)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human GFRAL-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Immobilised human GFRAL at 0.5 Mu g/ml (100 Mu l/Well). Dose response curve for human GDF15 with the EC50 of 9.4ng/ml. |
Target Information
| Gene Symbol | GFRAL |
| Gene ID | 389400 |
| Uniprot ID | GFRAL_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ser19-Glu351 |
Additional Info
| Post Translational Modifications | Cleaved and inactivated by MMP14, inhibiting the GDF15-GFRAL aversive response. |
| Function | Brainstem-restricted receptor for GDF15 hormone, which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses. The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future. The GDF15-GFRAL aversive response is triggered by stresses, such as anticancer drugs (camptothecin or cisplatin), cancers or drugs such as metformin. Upon interaction with its ligand, GDF15, mediates the GDF15-induced autophosphorylation and activation of the RET tyrosine kinase receptor, leading to activation of MAPK- and AKT- signaling pathways. Ligand-binding activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues. |
| Protein Name | Gdnf Family Receptor Alpha-Like |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Membrane ProteinExtracellular Side |
| Alternative Protein Names | Gdnf Family Receptor Alpha-Like proteinGFRAL proteinC6orf144 proteinUNQ9356 proteinPRO34128 protein |
Information sourced from Uniprot.org