Human GFAP (Glial fibrillary acidic protein) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human GFAP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 37.5pg/mL |
| Detection Limit | 62.5~4000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | GFAP |
| Gene ID | 2670 |
| Uniprot ID | GFAP_HUMAN |
| Specificity | This kit recognizes Human GFAP in samples. No significant cross-reactivity or interference between Human GFAP and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Expressed in cells lacking fibronectin. |
| Post Translational Modifications | Phosphorylated by PKN1. |
| Function | GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. |
| Protein Name | Glial Fibrillary Acidic ProteinGfap |
| Database Links | Reactome: R-HSA-1251985Reactome: R-HSA-9613829 |
| Cellular Localisation | CytoplasmAssociated With Intermediate Filaments |
| Alternative CLIA Names | Glial Fibrillary Acidic Protein CLIA kitGfap CLIA kitGFAP CLIA kit |
| Specificity | This kit recognizes Human GFAP in samples. No significant cross-reactivity or interference between Human GFAP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-106 | 98 |
| EDTA plasma(n=8) | 94-106 | 101 |
| Cell culture media(n=8) | 88-100 | 93 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 216.21 | 554.75 | 1858.10 | 196.81 | 548.78 | 1766.95 |
| Standard deviation | 23.24 | 55.25 | 139.73 | 19.62 | 39.90 | 158.67 |
| CV (%) | 10.75 | 9.96 | 7.52 | 9.97 | 7.27 | 8.98 |