Human GAD65 (GAD65) Antibody Pair (STJAP00775)
General Information
| Short Description | This GAD65 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R46-7A-6/R86-6O-6 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | GAD2 |
| Gene ID | 2572 |
| Uniprot ID | DCE2_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Post Translational Modifications | Phosphorylated.which does not affect kinetic parameters or subcellular location. Palmitoylated.which is required for presynaptic clustering. |
| Function | Catalyzes the production of GABA. |
| Protein Name | Glutamate Decarboxylase 265 Kda Glutamic Acid DecarboxylaseGad-65Glutamate Decarboxylase 65 Kda Isoform |
| Database Links | Reactome: R-HSA-888568Reactome: R-HSA-888590Reactome: R-HSA-9022927 |
| Cellular Localisation | CytoplasmCytosolCytoplasmic VesiclePresynaptic Cell MembraneLipid-AnchorGolgi Apparatus MembranePeripheral Membrane ProteinCytoplasmic SideAssociated To Cytoplasmic VesiclesIn NeuronsCytosolic Leaflet Of Golgi Membranes And Presynaptic Clusters |
| Alternative Antibody Pair Names | Glutamate Decarboxylase 2 antibody pair65 Kda Glutamic Acid Decarboxylase antibody pairGad-65 antibody pairGlutamate Decarboxylase 65 Kda Isoform antibody pairGAD2 antibody pairGAD65 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human GAD65 (GAD65) Antibody Pair (STJAP00775)
This antibody pair is designed to detect human GAD65, encoded by the GAD2 gene (UniProt Q05329). Reactivity has been confirmed for human samples only.
Both the capture and detection antibodies are rabbit monoclonal IgGs, with clone IDs R46-7A-6 and R86-6O-6 respectively. Both are supplied unconjugated at 1 mg/mL in 0.01M PBS, pH 7.2.
This pair has been validated for use in ELISA. No other assay formats are listed for this product.
Optimal working concentrations are determined by the end user and are not pre-defined for this pair. Titration experiments are recommended to establish the appropriate dilution for each antibody within the user's specific assay conditions.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles to maintain activity.
Both antibodies were raised in rabbit against a recombinant protein immunogen and purified by affinity chromatography. They are supplied as unconjugated IgG at 1 mg/mL in 0.01M PBS, pH 7.2.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.