| Host: | CHO cells |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human G-CSF-protein was developed from cho cells. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 Mu m filtered PBS solution pH7.0. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The ED (50) was determined by the dose-dependent proliferation of murine M-NFS-60 cells is ≤.1 ng/ml, corresponding to a specific activity of ≥ xunits/mg. NA |
| Gene Symbol: | CSF3 |
| Gene ID: | 1440 |
| Uniprot ID: | CSF3_HUMAN |
| Immunogen Region: | Mature chain |
| Immunogen: | Optimized DNA sequence encoding Human G-CSF mature chain was expressed in CHO Cells NA |
| Immunogen Sequence: | MAGPATQSPM KLMALQLLLW HSALWTVQEA TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCA TYKLCHPEEL VLLGHSLGIP WAPLSSCPSQ ALQLAGCLSQ LHSGLFLYQG LLQALEGISP ELGPTLDTLQ LDVADFATTI WQQMEELGMA PALQPTQGAM PAFASAFQRR AGGVLVASHL QSFLEVSYRV LRHLAQP NA |
| Post Translational Modifications | O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells). |
| Function | Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. |
| Protein Name | Granulocyte Colony-Stimulating FactorG-CsfPluripoietinFilgrastimLenograstim |
| Database Links | Reactome: R-HSA-449836Reactome: R-HSA-6783783Reactome: R-HSA-9674555Reactome: R-HSA-9705462 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Granulocyte Colony-Stimulating Factor proteinG-Csf proteinPluripoietin proteinFilgrastim proteinLenograstim proteinCSF3 proteinC17orf33 proteinGCSF protein |
Information sourced from Uniprot.org
