Human FZR1 protein (Recombinant) (N-His) (STJP007724)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human FZR1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | FZR1 |
| Gene ID | 51343 |
| Uniprot ID | FZR1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ser172-Arg496 |
Additional Info
| Post Translational Modifications | Acetylated. Deacetylated by SIRT2 at Lys-69 and Lys-159.deacetylation enhances the interaction of FZR1 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C). Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint. Phosphorylated by MAK. Ubiquitinated by the SCF(CCNF) E3 ubiquitin-protein ligase complex.leading to its degradation by the proteasome. |
| Function | Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR). |
| Protein Name | Fizzy-Related Protein HomologFzrCdc20-Like Protein 1Cdh1/Hct1 HomologHcdh1 |
| Database Links | Reactome: R-HSA-174084Reactome: R-HSA-174113Reactome: R-HSA-174178Reactome: R-HSA-176407Reactome: R-HSA-176408Reactome: R-HSA-176417Reactome: R-HSA-2559582Reactome: R-HSA-68867Reactome: R-HSA-69017Reactome: R-HSA-69656Reactome: R-HSA-8853884Reactome: R-HSA-9687136Reactome: R-HSA-983168 |
| Cellular Localisation | Isoform 2: NucleusIsoform 3: Cytoplasm |
| Alternative Protein Names | Fizzy-Related Protein Homolog proteinFzr proteinCdc20-Like Protein 1 proteinCdh1/Hct1 Homolog proteinHcdh1 proteinFZR1 proteinCDH1 proteinFYR proteinFZR proteinKIAA1242 protein |
Information sourced from Uniprot.org