Human Fucosyltransferase 7/FUT7 protein (Recombinant) (His-Tag) (STJP018721)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP018721
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General Information

Short DescriptionRecombinant-Human Fucosyltransferase 7/FUT7-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE/Denatured
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration0.25 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% Glycerol
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolFUT7
Gene ID2529
Uniprot IDFUT7_HUMAN
Accession NumberNP_004470
ImmunogenHuman
Immunogen Region37-342aa
Immunogen SequenceMGSSHHHHHH SSGLVPRGSH MGSPRGTPAP QPTITILVWH WPFTDQPPEL PSDTCTRYGI ARCHLSANRS LLASADAVVF HHRELQTRRS HLPLAQRPRG QPWVWASMES PSHTHGLSHL RGIFNWVLSY RRDSDIFVPY GRLEPHWGPS PPLPAKSRVA AWVVSNFQER QLRARLYRQL APHLRVDVFG RANGRPLCAS CLVPTVAQYR FYLSFENSQH RDYITEKF

Additional Info

Tissue Specificity Leukocytic/myeloid lineage cells.
Post Translational Modifications N-glycosylated.
Function Catalyzes the transfer of L-fucose, from a guanosine diphosphate-beta-L-fucose, to the N-acetyl glucosamine (GlcNAc) of a distal alpha2,3 sialylated lactosamine unit of a glycoprotein or a glycolipid-linked sialopolylactosamines chain through an alpha-1,3 glycosidic linkage and participates in the final fucosylation step in the biosynthesis of the sialyl Lewis X (sLe(x)), a carbohydrate involved in cell and matrix adhesion during leukocyte trafficking and fertilization. In vitro, also synthesizes sialyl-dimeric-Lex structures, from VIM-2 structures and both di-fucosylated and trifucosylated structures from mono-fucosylated precursors. However does not catalyze alpha 1-3 fucosylation when an internal alpha 1-3 fucosylation is present in polylactosamine chain and the fucosylation rate of the internal GlcNAc residues is reduced once fucose has been added to the distal GlcNAc. Also catalyzes the transfer of a fucose from GDP-beta-fucose to the 6-sulfated a(2,3)sialylated substrate to produce 6-sulfo sLex mediating significant L-selectin-dependent cell adhesion. Through sialyl-Lewis(x) biosynthesis, can control SELE- and SELP-mediated cell adhesion with leukocytes and allows leukocytes tethering and rolling along the endothelial tissue thereby enabling the leukocytes to accumulate at a site of inflammation. May enhance embryo implantation through sialyl Lewis X (sLeX)-mediated adhesion of embryo cells to endometrium. May affect insulin signaling by up-regulating the phosphorylation and expression of some signaling molecules involved in the insulin-signaling pathway through SLe(x) which is present on the glycans of the INSRR alpha subunit.
Protein Name Alpha-(1 -3-Fucosyltransferase 7
Fucosyltransferase 7
Fucosyltransferase Vii
Fuc-Tvii
Fuct-Vii
Galactoside 3-L-Fucosyltransferase
Selectin Ligand Synthase
Database Links Reactome: R-HSA-9037629
Cellular Localisation Golgi Apparatus
Golgi Stack Membrane
Single-Pass Type Ii Membrane Protein
Membrane-Bound Form In Trans Cisternae Of Golgi
Alternative Protein Names Alpha-(1 -3-Fucosyltransferase 7 protein
Fucosyltransferase 7 protein
Fucosyltransferase Vii protein
Fuc-Tvii protein
Fuct-Vii protein
Galactoside 3-L-Fucosyltransferase protein
Selectin Ligand Synthase protein
FUT7 protein

Information sourced from Uniprot.org

Citations

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