Human Fibrinogen alpha Chain (Fibrinogen alpha Chain) Antibody Pair (STJAP00934)
General Information
| Short Description | This Fibrinogen alpha Chain antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rat/Rat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | 2C2/9A2 |
| Isotype | IgG2a/IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | ELISA 1:1000 |
| Formulation | Liquid in PBS containing 0.09% sodium azide as a preservative./Liquid in PBS containing 0.01% thimerosal as a preservative. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | FGA |
| Gene ID | 2243 |
| Uniprot ID | FIBA_HUMAN |
| Immunogen | A synthesized peptide derived from FGA |
Additional Info
| Tissue Specificity | Detected in blood plasma (at protein level). |
| Post Translational Modifications | The alpha chain is normally not N-glycosylated, even though glycosylation at Asn-686 was observed when a fragment of the protein was expressed in insect cells. It is well known that heterologous expression of isolated domains can lead to adventitious protein modifications. Besides, glycosylation at Asn-686 is supported by large-scale glycoproteomics studies, but the evidence is still quite tenuous. Most likely, Asn-686 is not glycosylated in the healthy human body, or only with low efficiency. O-glycosylated. Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers. About one-third of the alpha chains in the molecules in blood were found to be phosphorylated. Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers. Phosphorylated by FAM20C in the extracellular medium. |
| Function | Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways. |
| Protein Name | Fibrinogen Alpha Chain Cleaved Into - Fibrinopeptide A - Fibrinogen Alpha Chain |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1236974Reactome: R-HSA-140875Reactome: R-HSA-166058Reactome: R-HSA-216083Reactome: R-HSA-354192Reactome: R-HSA-354194Reactome: R-HSA-372708Reactome: R-HSA-381426Reactome: R-HSA-5602498Reactome: R-HSA-5603041Reactome: R-HSA-5674135Reactome: R-HSA-5686938Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-8957275Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-977225 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Fibrinogen Alpha Chain Cleaved Into - Fibrinopeptide A - Fibrinogen Alpha Chain antibody pairFGA antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Fibrinogen alpha Chain (Fibrinogen alpha Chain) Antibody Pair (STJAP00934)
This antibody pair detects the human Fibrinogen alpha chain (FGA), with reactivity confirmed for human samples only. The target protein is referenced under UniProt accession P02671.
Both antibodies are supplied as affinity-purified monoclonal rat IgGs in liquid format at 1 mg/mL, and neither is conjugated. The capture antibody (clone 2C2, IgG2a) is formulated in PBS with 0.09% sodium azide, while the detection antibody (clone 9A2, IgG1) is formulated in PBS with 0.01% thimerosal.
This pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
The recommended dilution for both antibodies in ELISA is 1:1000, based on the supplied concentration of 1 mg/mL.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles to maintain stability.
Both antibodies were raised against a synthesized peptide derived from the human FGA protein. The host species for both clones is rat.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.