Human Fibrillarin/FBL protein (Recombinant) (His-Tag) (STJP016861)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Fibrillarin/FBL-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) containing 30% Glycerol, 1mM EDTA |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FBL |
| Gene ID | 2091 |
| Uniprot ID | FBRL_HUMAN |
| Accession Number | NP_001427 |
| Immunogen | Human |
| Immunogen Region | 83-321aa |
| Immunogen Sequence |
Additional Info
| Post Translational Modifications | Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36. By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA). Acetylated by CREBBP/CBP, preventing methylation of 'Gln-105' of histone H2A (H2AQ104me), without affecting rRNA methylation. Deacetylation by SIRT7 restores methylation of 'Gln-105' of histone H2A (H2AQ104me). |
| Function | S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes. U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. |
| Protein Name | Rrna 2'-O-Methyltransferase Fibrillarin34 Kda Nucleolar Scleroderma AntigenHistone-Glutamine MethyltransferaseU6 Snrna 2'-O-Methyltransferase Fibrillarin |
| Database Links | Reactome: R-HSA-6790901Reactome: R-HSA-6791226 |
| Cellular Localisation | NucleusNucleolusNucleoplasmFibrillar Region Of The Nucleolus |
| Alternative Protein Names | Rrna 2'-O-Methyltransferase Fibrillarin protein34 Kda Nucleolar Scleroderma Antigen proteinHistone-Glutamine Methyltransferase proteinU6 Snrna 2'-O-Methyltransferase Fibrillarin proteinFBL proteinFIB1 proteinFLRN protein |
Information sourced from Uniprot.org