| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human FGF1 at 5 Mu g/mL (100 Mu L/well) can bind Recombinant Human FGFR3 with a linear range of 0.5-1.5 Mu g/mL. |
| Gene Symbol: | FGFR3 |
| Gene ID: | 2261 |
| Uniprot ID: | FGFR3_HUMAN |
| Immunogen: | Recombinant Human FGFR-3/CD333 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Glu23-Gly375) of human FGFR3/CD333 (Accession #NP_000133.1) fused with an Fc, 6×His tag at the C-terminus. |
| Immunogen Sequence: | ESLGTEQRVVGRAAEVPGPE PGQQEQLVFGSGDAVELSCP PPGGGPMGPTVWVKDGTGLV PSERVLVGPQRLQVLNASHE DSGAYSCRQRLTQRVLCHFS VRVTDAPSSGDDEDGEDEAE DTGVDTGAPYWTRPERMDKK LLAVPAANTVRFRCPAAGNP TPSISWLKNGREFRGEHRIG GIKLRHQQWSLVMESVVPSD RGNYTCVVENKFGSIRQTYT LDVLERSPHRPILQAGLPA |
| Tissue Specificity | Expressed in brain, kidney and testis. Very low or no expression in spleen, heart, and muscle. In 20- to 22-week old fetuses it is expressed at high level in kidney, lung, small intestine and brain, and to a lower degree in spleen, liver, and muscle. Isoform 2 is detected in epithelial cells. Isoform 1 is not detected in epithelial cells. Isoform 1 and isoform 2 are detected in fibroblastic cells. |
| Post Translational Modifications | Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer. Phosphorylation at Tyr-724 is essential for stimulation of cell proliferation and activation of PIK3R1, STAT1 and MAP kinase signaling. Phosphorylation at Tyr-760 is required for interaction with PIK3R1 and PLCG1. Ubiquitinated. Is rapidly ubiquitinated after ligand binding and autophosphorylation, leading to receptor internalization and degradation. Subject to both proteasomal and lysosomal degradation. N-glycosylated in the endoplasmic reticulum. The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of PTPN11/SHP2, STAT1, STAT5A and STAT5B. Secreted isoform 3 retains its capacity to bind FGF1 and FGF2 and hence may interfere with FGF signaling. |
| Protein Name | Fibroblast Growth Factor Receptor 3 Fgfr-3 Cd Antigen Cd333 |
| Database Links | Reactome: R-HSA-109704 Reactome: R-HSA-1257604 Reactome: R-HSA-1839130 Reactome: R-HSA-190371 P22607-2 Reactome: R-HSA-190372 P22607-1 Reactome: R-HSA-2033515 Reactome: R-HSA-2219530 Reactome: R-HSA-5654227 Reactome: R-HSA-5654704 Reactome: R-HSA-5654706 Reactome: R-HSA-5654710 Reactome: R-HSA-5654732 Reactome: R-HSA-5655332 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-8853334 |
| Cellular Localisation | Isoform 1: Cell Membrane Single-Pass Type I Membrane Protein Cytoplasmic Vesicle Endoplasmic Reticulum The Activated Receptor Is Rapidly Internalized And Degraded Detected In Intracellular Vesicles After Internalization Of The Autophosphorylated Receptor Isoform 2: Cell Membrane Isoform 3: Secreted Isoform 4: Cell Membrane |
| Alternative Protein Names | Fibroblast Growth Factor Receptor 3 protein Fgfr-3 protein Cd Antigen Cd333 protein FGFR3 protein JTK4 protein |
Information sourced from Uniprot.org