Applications: | ELISA |
Reactivity: | Human |
Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: | 9.38pg/mL |
Detection Limit: | 15.63~1000pg/mL |
Short Description : | This FGF19 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: | 3.5h |
Detection: | Colormetric |
Gene Symbol: | FGF19 |
Gene ID: | 9965 |
Uniprot ID: | FGF19_HUMAN |
Specificity: | This kit recognizes Human FGF19 in samples. No significant cross-reactivity or interference between Human FGF19 and analogues was observed. |
Sample Type: | Serum, plasma and other biological fluids |
Function | Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. |
Protein Name | Fibroblast Growth Factor 19Fgf-19 |
Database Links | Reactome: R-HSA-109704Reactome: R-HSA-1257604Reactome: R-HSA-1307965Reactome: R-HSA-190322Reactome: R-HSA-2219530Reactome: R-HSA-5654228Reactome: R-HSA-5654712Reactome: R-HSA-5654719Reactome: R-HSA-5654720Reactome: R-HSA-5654733Reactome: R-HSA-5673001Reactome: R-HSA-6811558 |
Cellular Localisation | Secreted |
Alternative ELISA Names | Fibroblast Growth Factor 19 ELISA kitFgf-19 ELISA kitFGF19 ELISA kitUNQ334 ELISA kitPRO533 ELISA kit |
Specificity | This kit recognizes Human FGF19 in samples. No significant cross-reactivity or interference between Human FGF19 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 88-102 | 95 |
EDTA plasma (n=8) | 93-109 | 100 |
Cell culture media (n=8) | 84-96 | 91 |
Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
---|---|---|---|---|---|---|
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 48.71 | 118.24 | 371.84 | 45.18 | 125.53 | 341.83 |
Standard deviation | 2.52 | 5.27 | 14.76 | 2.81 | 6.68 | 11.08 |
CV (%) | 5.17 | 4.46 | 3.97 | 6.22 | 5.32 | 3.24 |