Human FBXO22 protein (Recombinant) (N-His) (STJP007631)

SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
STJP007631
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General Information

Short DescriptionRecombinant-Human FBXO22-N-His protein was developed from e.coli for the region N-His. For use in research applications.
ApplicationsELISA/Immunogen/SDS-PAGE/WB
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Dilution RangeReconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
FormulationLyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Storage InstructionUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt.

Target Information

Gene SymbolFBXO22
Gene ID26263
Uniprot IDFBX22_HUMAN
ImmunogenHomo sapiens (Human)
Immunogen RegionArg61-Lys403

Additional Info

Post Translational Modifications Phosphorylated by EIF2AK4 at Thr-127 causes cytoplasmic retention of FBXO22.
Function Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex that is implicated in the control of various cellular processes such as cell cycle control, transcriptional regulation, DNA damage repair, and apoptosis. Promotes the proteasome-dependent degradation of key sarcomeric proteins, such as alpha-actinin (ACTN2) and filamin-C (FLNC), essential for maintenance of normal contractile function. Acts as a key regulator of histone methylation marks namely H3K9 and H3K36 methylation through the regulation of histone demethylase KDM4A protein levels. In complex with KDM4A, also regulates the abundance of TP53 by targeting methylated TP53 for degradation at the late senescent stage. Under oxidative stress, promotes the ubiquitination and degradation of BACH1. Mechanistically, reactive oxygen species (ROS) covalently modify cysteine residues on the bZIP domain of BACH1, leading to its release from chromatin and making it accessible to FBXO22. Upon amino acid depletion, mediates 'Lys-27'-linked ubiquitination of MTOR and thereby inhibits substrate recruitment to mTORC1. Also inhibits SARS-CoV-2 replication by inducing NSP5 degradation.
Protein Name F-Box Only Protein 22
F-Box Protein Fbx22p44
Database Links Reactome: R-HSA-8951664
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Myofibril
Sarcomere
Z Line
Amino Acid Depletion Lead To A Time-Dependent Increase Of Fbxo22 In The Cytoplasm
Alternative Protein Names F-Box Only Protein 22 protein
F-Box Protein Fbx22p44 protein
FBXO22 protein
FBX22 protein

Information sourced from Uniprot.org

Citations

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