Human FABP2 (Fatty acid-binding protein, intestinal) Sandwich ELISA Kit (STJE0002023)

SPECIFICATIONS
STJE0002023
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General Information

Short DescriptionThis FABP2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
ApplicationsELISA
ReactivityHuman
Sensitivity0.09ng/mL
Detection Limit0.16~10ng/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionColormetric

Target Information

Gene SymbolFABP2
Gene ID2169
Uniprot IDFABPI_HUMAN
SpecificityThis kit recognizes Human IFABP/FABP2 in samples. No significant cross-reactivity or interference between Human IFABP/FABP2 and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Function FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
Protein Name Fatty Acid-Binding Protein - Intestinal
Fatty Acid-Binding Protein 2
Intestinal-Type Fatty Acid-Binding Protein
I-Fabp
Database Links Reactome: R-HSA-163560
Cellular Localisation Cytoplasm
Alternative ELISA Names Fatty Acid-Binding Protein - Intestinal ELISA kit
Fatty Acid-Binding Protein 2 ELISA kit
Intestinal-Type Fatty Acid-Binding Protein ELISA kit
I-Fabp ELISA kit
FABP2 ELISA kit
FABPI ELISA kit
Specificity This kit recognizes Human IFABP/FABP2 in samples. No significant cross-reactivity or interference between Human IFABP/FABP2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-107 98
EDTA plasma (n=8) 94-107 101
Cell culture media (n=8) 89-100 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.55 1.11 3.94 0.50 1.11 4.25
Standard deviation 0.03 0.07 0.13 0.03 0.06 0.16
CV (%) 5.45 6.31 3.30 6.00 5.41 3.76

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