| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.1IU/mL |
| Detection Limit: | 0.16~10IU/mL |
| Short Description : | This F8 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | F8 |
| Gene ID: | 2157 |
| Uniprot ID: | FA8_HUMAN |
| Specificity: | This kit recognizes Human F8 in samples. No significant cross-reactivity or interference between Human F8 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa. |
| Protein Name | Coagulation Factor ViiiAntihemophilic FactorAhfProcoagulant Component Cleaved Into - Factor Viiia Heavy Chain - 200 Kda Isoform - Factor Viiia Heavy Chain - 92 Kda Isoform - Factor Viii B Chain - Factor Viiia Light Chain |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-140837Reactome: R-HSA-140875Reactome: R-HSA-163841Reactome: R-HSA-204005Reactome: R-HSA-5694530Reactome: R-HSA-9672383Reactome: R-HSA-9672387Reactome: R-HSA-9672391Reactome: R-HSA-9672393Reactome: R-HSA-9672395Reactome: R-HSA-9672396Reactome: R-HSA-9672397Reactome: R-HSA-9673202Reactome: R-HSA-9674519 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative ELISA Names | Coagulation Factor Viii ELISA kitAntihemophilic Factor ELISA kitAhf ELISA kitProcoagulant Component Cleaved Into - Factor Viiia Heavy Chain - 200 Kda Isoform - Factor Viiia Heavy Chain - 92 Kda Isoform - Factor Viii B Chain - Factor Viiia Light Chain ELISA kitF8 ELISA kitF8C ELISA kit |
| Specificity | This kit recognizes Human F8 in samples. No significant cross-reactivity or interference between Human F8 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 99-109 | 102 |
| EDTA plasma (n=8) | 87-96 | 90 |
| Cell culture media (n=8) | 96-108 | 104 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (IU/mL) | 0.44 | 1.19 | 4.01 | 0.49 | 1.02 | 4.03 |
| Standard deviation | 0.02 | 0.06 | 0.17 | 0.03 | 0.05 | 0.21 |
| CV (%) | 4.55 | 5.04 | 4.24 | 6.12 | 4.90 | 5.21 |