| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69pg/mL |
| Detection Limit: |
7.81~500pg/mL |
| Short Description: |
This human ESM1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
ESM1 |
| Gene ID: |
11082 |
| Uniprot ID: |
ESM1_HUMAN |
| Specificity: |
This kit recognizes Human ESM1 in samples. No significant cross-reactivity or interference between Human ESM1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney. |
| Post Translational Modifications | May contain intrachain disulfide bonds. O-glycosylated.contains chondroitin sulfate and dermatan sulfate. |
| Function | Involved in angiogenesis.promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions. |
| Protein Name | Endothelial Cell-Specific Molecule 1Esm-1 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Endothelial Cell-Specific Molecule 1 CLIA kitEsm-1 CLIA kitESM1 CLIA kit |
| Specificity | This kit recognizes Human ESM1 in samples. No significant cross-reactivity or interference between Human ESM1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 99-115 | 105 |
| EDTA plasma(n=8) | 90-104 | 95 |
| Cell culture media(n=8) | 98-113 | 104 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 23.54 | 45.15 | 245.07 | 22.75 | 43.28 | 231.41 |
| Standard deviation | 3.02 | 3.90 | 19.88 | 2.81 | 3.14 | 25.41 |
| CV (%) | 12.83 | 8.64 | 8.11 | 12.35 | 7.26 | 10.98 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
