• Single CD34-LSK cells were sorted into 96-well microtiter plates containing the SF-O3 medium supplemented with 10% FBS and multiple cytokines (10 ng/ml SCF, 10 ng/ml TPO, 10 ng/ml IL-3, and 3 u/ml EPO) and allowed to form colonies.
  • The effects of SNS-032, perifosine, or combination on the leukemia colony formation (CFU-L) in methylcellulose medium were examined using leukemic colony assay as previously described [3) in 600 Mu L of methylcellulose solution were incubated in the presence of the agents or an equivalent amount of medium at 37°C in a humidified atmosphere with 5% CO2. Primary leukemic cells were cultured in methylcellulose medium containing recombinant human (rh) stem cell factor (SCF) , granulocyte macrophage-colony-stimulating factor (GM-CSF) , and interleukin 3 (IL-3) at 2 × 104 cells/dish. After 7 days, CFU-Ls that contain >40 cells were scored manually under a light microscope. For colony assay of human normal bone marrow cells, 3 U/mL rh erythropoietin, 50 ng/mL rhSCF, 30 ng/mL rhGM-CSF, and 10 ng/mL rhIL-3 were added to the methylcellulose medium. The colonies were counted under a microscope on day 12 of culture.
  • celltype NA >Leukemic cell lines, MNCs from the patients with, or healthy controls were infected with or without the indicated viruses (50 MOI) and seeded in triplicate in a mixture containing 1. 35% methylcellulose in medium NA >Iscove NA s medium NA >modified medium NA >Dulbecco NA s medium (IMDM;) supplemented with 20% fetal bovine serum and10−4 M 2-Mercaptoethanol. For colony assay of bone marrow cells, 3 U/mL recombinant human (rh) erythropoietin, 50 ng/mL rh stem cell factor, 30 ng/mL rh granulocyte macrophage–colony-stimulating factor, and 10 ng/mL rh interleukin-3 were added to the methylcellulose medium. The colonies were evaluated under a microscope on day 12 of culture. In contrast, leukemic cell lines were seeded in cytokine-free mixture as described previously [

Human Erythropoietin-alpha protein (Recombinant) (STJP000331)

SKU:
STJP000331

£136.50 - £1,546.50
Current Stock:
Host: CHO cells
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human Erythropoietin-alpha-protein was developed from cho cells. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 Mu m filtered PBS solution pH.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA
Immunoreactivity: Activity was determined by the dose-dependent proliferation assay using a factor-dependent human erythroleukemic cell line TF-1 and was found to be.8x105 IU/mg. NA
Gene Symbol: EPO
Gene ID: 2056
Uniprot ID: EPO_HUMAN
Immunogen Region: Mature chain
Immunogen: Optimized DNA sequence encoding Human EPO mature chain was expressed in CHO cells. NA
Immunogen Sequence: MGVHECPAWL WLLLSLLSLP LGLPVLGAPP RLICDSRVLE RYLLEAKEAE NITTGCAEHC SLNENITVPD TKVNFYAWKR MEVGQQAVEV WQGLALLSEA VLRGQALLVN SSQPWEPLQL HVDKAVSGLR SLTTLLRALG AQKEAISPPD AASAAPLRTI TADTFRKLFR VYSNFLRGKL KLYTGEACRT GDR NA
Tissue Specificity Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Function Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
Protein Name Erythropoietin
Epoetin
Database Links Reactome: R-HSA-1234158
Reactome: R-HSA-9006335
Reactome: R-HSA-9027276
Reactome: R-HSA-9027277
Reactome: R-HSA-9027283
Reactome: R-HSA-9027284
Cellular Localisation Secreted
Alternative Protein Names Erythropoietin protein
Epoetin protein
EPO protein

Information sourced from Uniprot.org

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