Human ERO1A protein (Recombinant) (C-His) (STJP013736)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human ERO1A-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a 0.22 Mu m filtered solution in 20mM PB, 150mM NaCl, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | < 1 EU/µg as determined by LAL test. |
Target Information
| Gene Symbol | ERO1A |
| Gene ID | 30001 |
| Uniprot ID | ERO1A_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Glu24-His468 |
Additional Info
| Post Translational Modifications | N-glycosylated. The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center. The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397. The regulatory Cys-99/Cys-104 disulfide bond stabilizes the other regulatory bond Cys-94/Cys-131. Phosphorylated on Ser-145 by FAM20C in the Golgi which increases its enzymatic activity. Phosphorylation is induced by lactation. It is also induced by hypoxia and reductive stress. |
| Function | Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. |
| Protein Name | Ero1-Like Protein AlphaEro1-LEro1-L-AlphaEndoplasmic Oxidoreductin-1-Like ProteinEndoplasmic Reticulum Oxidoreductase AlphaOxidoreductin-1-L-Alpha |
| Database Links | Reactome: R-HSA-3299685 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinLumenal SideGolgi Apparatus LumenSecretedCell ProjectionDendriteThe Association With Erp44 Is Essential For Its Retention In The Endoplasmic ReticulumIn NeuronsIt Localizes To Dendrites |
| Alternative Protein Names | Ero1-Like Protein Alpha proteinEro1-L proteinEro1-L-Alpha proteinEndoplasmic Oxidoreductin-1-Like Protein proteinEndoplasmic Reticulum Oxidoreductase Alpha proteinOxidoreductin-1-L-Alpha proteinERO1A proteinERO1L proteinUNQ434 proteinPRO865 protein |
Information sourced from Uniprot.org