Human EPO receptor (EPOR) protein (Recombinant) (Fc-Tag) (STJP000466)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenDNA sequence encoding the extracellular domain of Human EPOR including a C-terminal human IgG1-Fc tag was expressed in HEK293 Cells. NA
General Information
| Short Description | Recombinant-Human EPO receptor (EPOR)-Fc-Tag protein was developed from hek293 and has a target region of Fc-Tag. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 ยตm filtered PBS solution pH7.4. |
| Storage Instruction | The lyophilized protein is stable for at least 1 year from date of receipt at-20ยฐC. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ยตg (1EU/ยตg). NA |
| Immunoreactivity | The activity was determined by its ability to bind in functional ELISA assay. NA |
Target Information
| Gene Symbol | EPOR |
| Gene ID | 2057 |
| Uniprot ID | EPOR_HUMAN |
| Immunogen | DNA sequence encoding the extracellular domain of Human EPOR including a C-terminal human IgG1-Fc tag was expressed in HEK293 Cells. NA |
| Immunogen Region | ECD |
| Immunogen Sequence | APPPNLPDPK FESKAALLAA RGPEELLCFT ERLEDLVCFW EEAASAGVGP GNYSFSYQLE DEPWKLCRLH QAPTARGAVR FWCSLPTADT SSFVPLELRV TAASGAPRYH RVIHINEVVL LDAPVGLVAR LADESGHVVL RWLPPPETPM TSHIRYEVDV SAGNGAGSVQ RVEILEGRTE CVLSNLRGRT RYTFAVRARM AEPSFGGFWS AWSEPVSLLT NA |
Additional Info
| Tissue Specificity | Erythroid cells and erythroid progenitor cells. Isoform EPOR-F: Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S: Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T: Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells. |
| Post Translational Modifications | On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site. Ubiquitinated by the ECS(SOCS2) complex following ligand-binding and phosphorylation by JAK2, leading to its degradation by the proteasome. Regulation by the ECS(SOCS2) complex acts as a negative feedback loop of erythropoietin-mediated signaling pathway. Ubiquitination at Lys-281 mediates receptor internalization, whereas ubiquitination at Lys-453 promotes trafficking of activated receptors to the lysosomes for degradation. Ubiquitinated by NOSIP.appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells. |
| Function | Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase. Isoform EPOR-T: Acts as a dominant-negative receptor of EPOR-mediated signaling. |
| Protein Name | Erythropoietin ReceptorEpo-R |
| Database Links | Reactome: R-HSA-9006335Reactome: R-HSA-9027276Reactome: R-HSA-9027277Reactome: R-HSA-9027283Reactome: R-HSA-9027284 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinIsoform Epor-S: SecretedSecreted And Located To The Cell Surface |
| Alternative Protein Names | Erythropoietin Receptor proteinEpo-R proteinEPOR protein |
Information sourced from Uniprot.org