| Host: | Cereal grain NA |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Recombinant-Human Epidermal Growth Factor-Plant derived protein was developed from cereal grain and has a target region of Plant derived. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.2 Mu m filtered concentrated (1mg/ml) solution in PBS, pH 7.2. NA |
| Dilution Range: | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. NA |
| Storage Instruction: | The lyophilized protein is stable for at least 2 years from date of receipt at-20°C C. Upon reconstitution, this cytokine can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detect |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Gene Symbol: | EGF |
| Gene ID: | 1950 |
| Uniprot ID: | EGF_HUMAN |
| Immunogen Region: | Mature chain |
| Immunogen: | DNA sequence encoding Human EGF mature chain was expressed in cereal grain. NA |
| Post Translational Modifications | O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). |
| Function | EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro. |
| Protein Name | Pro-Epidermal Growth FactorEgf Cleaved Into - Epidermal Growth FactorUrogastrone |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1227986Reactome: R-HSA-1236382Reactome: R-HSA-1236394Reactome: R-HSA-1250196Reactome: R-HSA-1251932Reactome: R-HSA-1257604Reactome: R-HSA-177929Reactome: R-HSA-179812Reactome: R-HSA-180292Reactome: R-HSA-180336Reactome: R-HSA-182971Reactome: R-HSA-1963640Reactome: R-HSA-1963642Reactome: R-HSA-212718Reactome: R-HSA-2219530Reactome: R-HSA-5637810Reactome: R-HSA-5638303Reactome: R-HSA-5673001Reactome: R-HSA-6785631Reactome: R-HSA-6811558Reactome: R-HSA-8847993Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8863795Reactome: R-HSA-9009391Reactome: R-HSA-9013507Reactome: R-HSA-9634638Reactome: R-HSA-9664565Reactome: R-HSA-9665348Reactome: R-HSA-9665686 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Pro-Epidermal Growth Factor proteinEgf Cleaved Into - Epidermal Growth Factor proteinUrogastrone proteinEGF protein |
Information sourced from Uniprot.org
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