| Short Description : | Recombinant-Human EphB4/HTK-C-hFc & His protein was developed in hek293 cells using the region Met 1-Ala 539. For use in research applications. |
| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized Human EFNB2 at 0.5 Mu g/mL (100 Mu L/well) can bind Human EPHB4 with a linear range of 6-400 pg/mL. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | EPHB4 |
| Gene ID: | 2050 |
| Uniprot ID: | EPHB4_HUMAN |
| Immunogen: | Recombinant Human EphB4/HTK Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met 1-Ala 539) of human EphB4/HTK (Accession #NP_004435.3) fused with a Fc, 6×His tag at the C-terminus. |
| Immunogen Region: | Met 1-Ala 539 |
| Immunogen Sequence: | MELRVLLCWASLAAALEETL LNTKLETADLKWVTFPQVDG QWEELSGLDEEQHSVRTYEV CDVQRAPGQAHWLRTGWVPR RGAVHVYATLRFTMLECLSL PRAGRSCKETFTVFYYESDA DTATALTPAWMENPYIKVDT VAAEHLTRKRPGAEATGKVN VKTLRLGPLSKAGFYLAFQD QGACMALLSLHLFYKKCAQL TVNLTRFPETVPRELVVPVA GSCVVDAVPAPGPSPSLYC |
| Post Translational Modifications | Phosphorylated.autophosphorylation is stimulated by EFNB2. |
| Function | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. |
| Protein Name | Ephrin Type-B Receptor 4Hepatoma Transmembrane KinaseTyrosine-Protein Kinase Tyro11 |
| Database Links | Reactome: R-HSA-2682334Reactome: R-HSA-3928662Reactome: R-HSA-3928664Reactome: R-HSA-3928665 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Ephrin Type-B Receptor 4 proteinHepatoma Transmembrane Kinase proteinTyrosine-Protein Kinase Tyro11 proteinEPHB4 proteinHTK proteinMYK1 proteinTYRO11 protein |
Information sourced from Uniprot.org
