Human EphA7/EHK-3 protein (Recombinant) (C-His) (STJP002731)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human EphA7/EHK-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Val555) of human EphA7 (Accession #NP_004431) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human EphA7/EHK-3-C-His protein was developed in hek293 cells using the region Met1-Val555. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Human EphA7 at 0.5 Mu g/mL (100 Mu L/well) can bind Human EFNA4 with a linear range of 0.039-0.942 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | EPHA7 |
| Gene ID | 2045 |
| Uniprot ID | EPHA7_HUMAN |
| Immunogen | Recombinant Human EphA7/EHK-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Val555) of human EphA7 (Accession #NP_004431) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Met1-Val555 |
| Immunogen Sequence | MVFQTRYPSWIILCYIWLLR FAHTGEAQAAKEVLLLDSKA QQTELEWISSPPNGWEEISG LDENYTPIRTYQVCQVMEPN QNNWLRTNWISKGNAQRIFV ELKFTLRDCNSLPGVLGTCK ETFNLYYYETDYDTGRNIRE NLYVKIDTIAADESFTQGDL GERKMKLNTEVREIGPLSKK GFYLAFQDVGACIALVSVKV YYKKCWSIIENLAIFPDTVT GSEFSSLVEVRGTCVSSAE |
Additional Info
| Post Translational Modifications | Phosphorylated. |
| Function | Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 and MAPK3 which are phosphorylated upon activation of EPHA7. |
| Protein Name | Ephrin Type-A Receptor 7Eph Homology Kinase 3Ehk-3Eph-Like Kinase 11Ek11Hek11 |
| Database Links | Reactome: R-HSA-2682334Reactome: R-HSA-3928663Reactome: R-HSA-3928665 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Ephrin Type-A Receptor 7 proteinEph Homology Kinase 3 proteinEhk-3 proteinEph-Like Kinase 11 proteinEk11 proteinHek11 proteinEPHA7 proteinEHK3 proteinHEK11 protein |
Information sourced from Uniprot.org