Human EPHA4 protein (Recombinant) (Tag free) (STJP013372)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human EPHA4-Tag free protein was developed from mammalian cells and has a target region of Tag free. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a 0.22 Mu m filtered solution in PBS, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8ยฐC for one week. Store at-20 to-80ยฐC for twelve months from the date of receipt. |
| Endotoxin | < 1 EU/ยตg as determined by LAL test. |
Target Information
| Gene Symbol | EPHA4 |
| Gene ID | 2043 |
| Uniprot ID | EPHA4_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Val20-Thr547 |
Additional Info
| Function | Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, also plays a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium. During development of the cochlear organ of Corti, regulates pillar cell separation by forming a ternary complex with ADAM10 and CADH1 which facilitates the cleavage of CADH1 by ADAM10 and disruption of adherens junctions. Phosphorylates CAPRIN1, promoting CAPRIN1-dependent formation of a membraneless compartment. |
| Protein Name | Ephrin Type-A Receptor 4Eph-Like Kinase 8Ek8Hek8Tyrosine-Protein Kinase Tyro1Tyrosine-Protein Kinase Receptor Sek |
| Database Links | Reactome: R-HSA-2682334Reactome: R-HSA-3928663Reactome: R-HSA-3928665Reactome: R-HSA-9824272 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionAxonDendritePostsynaptic Density MembraneEarly EndosomeCell JunctionAdherens JunctionClustered Upon Activation And Targeted To Early Endosome |
| Alternative Protein Names | Ephrin Type-A Receptor 4 proteinEph-Like Kinase 8 proteinEk8 proteinHek8 proteinTyrosine-Protein Kinase Tyro1 proteinTyrosine-Protein Kinase Receptor Sek proteinEPHA4 proteinHEK8 proteinSEK proteinTYRO1 protein |
Information sourced from Uniprot.org