This human ENPP7 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications
CLIA
Reactivity
Human
Sensitivity
46.88pg/mL
Detection Limit
78.13~5000pg/mL
Note
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
This kit recognizes Human Alk-Smase in samples. No significant cross-reactivity or interference between Human Alk-Smase and analogues was observed.
Sample Type
Serum, plasma and other biological fluids
Additional Info
Tissue Specificity
Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon.
Post Translational Modifications
N-glycosylated.required for activity and transport to the plasma membrane.
Function
Choline-specific phosphodiesterase that hydrolyzes sphingomyelin releasing the ceramide and phosphocholine and therefore is involved in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption in the gastrointestinal tract. Also has phospholipase C activity and can also cleave phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF) leading to its inactivation. Does not have nucleotide pyrophosphatase activity. May promote cholesterol absorption by affecting the levels of sphingomyelin derived from either diet or endogenous sources, in the intestinal lumen.
Protein Name
Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 7E-Npp 7Npp-7Alkaline Sphingomyelin PhosphodiesteraseIntestinal Alkaline SphingomyelinaseAlk-Smase
Cell MembraneSingle-Pass Type I Membrane ProteinThe Catalytic Domain Is Released Into The Extracellular Medium When Cells Are Treated With TrypsinLocalized At The Surface Of The Microvillar Membrane In Small Intestine EnterocytesAnd In Endosome-Like Structures Situated Beneath The Microvillar MembraneAnd In Golgi Complex
