| Host: | E.coli |
| Applications: | SDS-PAGE/Enzyme Activity |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human Enolase 3/ENO3-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Formulation: | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% Glycerol, 0.1M NaCl |
| Concentration: | 0.5 mg/mL |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | Specific activity is > 5, 000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C. |
| Gene Symbol: | ENO3 |
| Gene ID: | 2027 |
| Uniprot ID: | ENOB_HUMAN |
| Immunogen Region: | 1-434aa |
| Accession Number: | AAH17249 |
| Immunogen: | Human |
| Immunogen Sequence: |
| Tissue Specificity | The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. |
| Function | Glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. Appears to have a function in striated muscle development and regeneration. |
| Protein Name | Beta-Enolase2-Phospho-D-Glycerate Hydro-LyaseEnolase 3Muscle-Specific EnolaseMseSkeletal Muscle Enolase |
| Database Links | Reactome: R-HSA-70171Reactome: R-HSA-70263 |
| Cellular Localisation | CytoplasmLocalized To The Z LineSome Colocalization With Ckm At M-Band |
| Alternative Protein Names | Beta-Enolase protein2-Phospho-D-Glycerate Hydro-Lyase proteinEnolase 3 proteinMuscle-Specific Enolase proteinMse proteinSkeletal Muscle Enolase proteinENO3 protein |
Information sourced from Uniprot.org