Human EGF (EGF) Antibody Pair (STJAP00667)
General Information
| Short Description | This EGF antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | 6B8/5B3 |
| Isotype | IgG1/IgG2a |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000ELISA 1:1000 |
| Formulation | Liquid in PBS containing 0.09% sodium azide as a preservative. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | EGF |
| Gene ID | 1950 |
| Uniprot ID | EGF_HUMAN |
| Immunogen | Recombinant human EGF |
Additional Info
| Tissue Specificity | Expressed in kidney, salivary gland, cerebrum and prostate. |
| Post Translational Modifications | O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). |
| Function | EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro. |
| Protein Name | Pro-Epidermal Growth FactorEgf Cleaved Into - Epidermal Growth FactorUrogastrone |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1227986Reactome: R-HSA-1236382Reactome: R-HSA-1236394Reactome: R-HSA-1250196Reactome: R-HSA-1251932Reactome: R-HSA-1257604Reactome: R-HSA-177929Reactome: R-HSA-179812Reactome: R-HSA-180292Reactome: R-HSA-180336Reactome: R-HSA-182971Reactome: R-HSA-1963640Reactome: R-HSA-1963642Reactome: R-HSA-212718Reactome: R-HSA-2219530Reactome: R-HSA-5637810Reactome: R-HSA-5638303Reactome: R-HSA-5673001Reactome: R-HSA-6785631Reactome: R-HSA-6811558Reactome: R-HSA-8847993Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8863795Reactome: R-HSA-9009391Reactome: R-HSA-9013507Reactome: R-HSA-9634638Reactome: R-HSA-9664565Reactome: R-HSA-9665348Reactome: R-HSA-9665686Reactome: R-HSA-9725554Reactome: R-HSA-9818030Reactome: R-HSA-9925561 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Pair Names | Pro-Epidermal Growth Factor antibody pairEgf Cleaved Into - Epidermal Growth Factor antibody pairUrogastrone antibody pairEGF antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human EGF (EGF) Antibody Pair (STJAP00667)
This antibody pair is validated for the detection of human EGF (Epidermal Growth Factor, UniProt P01133) and has confirmed reactivity to human samples only. Both antibodies were raised against recombinant human EGF.
Both the capture and detection antibodies are mouse monoclonal IgG antibodies supplied unconjugated at 1 mg/mL in PBS containing 0.09% sodium azide, and both are affinity purified. Clone 6B8 is IgG1 and clone 5B3 is IgG2a.
This pair is validated for use in ELISA at a recommended dilution of 1:1000. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
Yes, both antibodies are supplied unconjugated, so researchers will need to conjugate the detection antibody to an appropriate reporter, such as biotin or HRP, prior to use in a sandwich ELISA format.
Both antibodies should be stored at 4 degrees C for short-term use. For long-term storage, aliquot and store at -20 degrees C. Repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.