Human EEF1A1 protein (Recombinant) (N-His) (STJP005056)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human EEF1A1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | EEF1A1 |
| Gene ID | 1915 |
| Uniprot ID | EF1A1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Pro241 |
Additional Info
| Function | Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome. The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation. Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1. Also plays a role in cytoskeleton organization by promoting actin bundling. (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection. |
| Protein Name | Elongation Factor 1-Alpha 1Ef-1-Alpha-1Elongation Factor TuEf-TuEukaryotic Elongation Factor 1 A-1Eef1a-1Leukocyte Receptor Cluster Member 7 |
| Database Links | Reactome: R-HSA-156842Reactome: R-HSA-156902Reactome: R-HSA-3371511Reactome: R-HSA-6798695Reactome: R-HSA-8876725Reactome: R-HSA-9613829Reactome: R-HSA-9735869 |
| Cellular Localisation | CytoplasmNucleusNucleolusCell MembraneColocalizes With Dlc1 At Actin-Rich Regions In The Cell PeripheryTranslocates Together With Zpr1 From The Cytoplasm To The Nucleus And Nucleolus After Treatment With MitogensLocalization At The Cell Membrane Depends On Eef1a1 Phosphorylation Status And The Presence Of Ppp1r16b |
| Alternative Protein Names | Elongation Factor 1-Alpha 1 proteinEf-1-Alpha-1 proteinElongation Factor Tu proteinEf-Tu proteinEukaryotic Elongation Factor 1 A-1 proteinEef1a-1 proteinLeukocyte Receptor Cluster Member 7 proteinEEF1A1 proteinEEF1A proteinEF1A proteinLENG7 protein |
Information sourced from Uniprot.org