Human eEF-2K/EEF2K protein (Recombinant) (His-Tag) (STJP018932)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human eEF-2K/EEF2K-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EEF2K |
| Gene ID | 29904 |
| Uniprot ID | EF2K_HUMAN |
| Accession Number | NP_037434.1 |
| Immunogen | Human |
| Immunogen Region | 1-725aa |
| Immunogen Sequence |
Additional Info
| Post Translational Modifications | Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis. |
| Function | Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced. |
| Protein Name | Eukaryotic Elongation Factor 2 KinaseEef-2 KinaseEef-2kCalcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase |
| Database Links | Reactome: R-HSA-166208 |
| Cellular Localisation | |
| Alternative Protein Names | Eukaryotic Elongation Factor 2 Kinase proteinEef-2 Kinase proteinEef-2k proteinCalcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase proteinEEF2K protein |
Information sourced from Uniprot.org