Human DNMT3L protein (Recombinant) (His-Tag) (STJP018792)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human DNMT3L-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% Glycerol, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DNMT3L |
| Gene ID | 29947 |
| Uniprot ID | DNM3L_HUMAN |
| Accession Number | NP_787063 |
| Immunogen | Human |
| Immunogen Region | 1-386aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Expressed at low levels in several tissues including testis, ovary, and thymus. |
| Function | Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context. Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain. Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3. Plays a key role in embryonic stem cells and germ cells. In germ cells, required for the methylation of imprinted loci together with DNMT3A. In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization. Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation. While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes. Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes. Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression. |
| Protein Name | DnaCytosine-5-Methyltransferase 3-Like |
| Database Links | Reactome: R-HSA-5334118Reactome: R-HSA-9845323 |
| Cellular Localisation | Nucleus |
| Alternative Protein Names | Dna proteinCytosine-5-Methyltransferase 3-Like proteinDNMT3L protein |
Information sourced from Uniprot.org