Human DNM1 protein (Recombinant) (N-His) (STJP007772)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human DNM1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | DNM1 |
| Gene ID | 1759 |
| Uniprot ID | DYN1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Arg271 |
Additional Info
| Function | Catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission and participates in many forms of endocytosis, such as clathrin-mediated endocytosis or synaptic vesicle endocytosis as well as rapid endocytosis (RE). Associates to the membrane, through lipid binding, and self-assembles into rings and stacks of interconnected rings through oligomerization to form a helical polymer around the vesicle membrane leading to constriction of invaginated coated pits around their necks. Self-assembly of the helical polymer induces membrane tubules narrowing until the polymer reaches a length sufficient to trigger GTP hydrolysis. Depending on the curvature imposed on the tubules, membrane detachment from the helical polymer upon GTP hydrolysis can cause spontaneous hemifission followed by complete fission. May play a role in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells through its activation by dephosphorylation via the signaling downstream of EGFR. Controls vesicle size at a step before fission, during formation of membrane pits, at hippocampal synapses. Controls plastic adaptation of the synaptic vesicle recycling machinery to high levels of activity. Mediates rapid endocytosis (RE), a Ca(2+)-dependent and clathrin- and K(+)-independent process in chromaffin cells. Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Through its interaction with DNAJC6, acts during the early steps of clathrin-coated vesicle (CCV) formation. |
| Protein Name | Dynamin-1DynaminDynamin I |
| Database Links | Reactome: R-HSA-166016Reactome: R-HSA-177504Reactome: R-HSA-190873Reactome: R-HSA-196025Reactome: R-HSA-2132295Reactome: R-HSA-3928665Reactome: R-HSA-437239Reactome: R-HSA-8856828 |
| Cellular Localisation | Cell MembraneMembraneClathrin-Coated PitCytoplasmic VesiclePresynapseSecretory VesicleChromaffin GranuleAssociated To The Membrane In A Helical Polymer Shape In A Gtp Bound StateTransiently Recruited To Endocytic Clathrin-Coated Pits (Ccps) At A Late Stage Of Clathrin-Coated Vesicle (Ccv) Formation |
| Alternative Protein Names | Dynamin-1 proteinDynamin proteinDynamin I proteinDNM1 proteinDNM protein |
Information sourced from Uniprot.org