Human DNASE1L3 protein (Recombinant) (N-His) (STJP016300)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human DNASE1L3-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for further information. |
Target Information
| Gene Symbol | DNASE1L3 |
| Gene ID | 1776 |
| Uniprot ID | DNSL3_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met21-Ser305 |
Additional Info
| Post Translational Modifications | Poly-ADP-ribosylated by PARP1. ADP-ribosylation negatively regulates enzymatic activity during apoptosis. |
| Function | Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends. Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA. Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis. The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells. Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity. Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation. |
| Protein Name | Deoxyribonuclease GammaDnase GammaDnase I Homolog Protein Dhp2Deoxyribonuclease I-Like 3Dnase I-Like 3Liver And Spleen DnaseLs-DnaseLsd |
| Database Links | |
| Cellular Localisation | NucleusEndoplasmic ReticulumSecretedTranslocates From The Endoplasmic Reticulum To The Nucleus During ApoptosisContradictory Reports Exist About The Subcellular Localization Under Normal Physiological ConditionsUnder Conditions Of Cell DeathMay Diffuse And/Or Be Actively Transported To The Nucleus |
| Alternative Protein Names | Deoxyribonuclease Gamma proteinDnase Gamma proteinDnase I Homolog Protein Dhp2 proteinDeoxyribonuclease I-Like 3 proteinDnase I-Like 3 proteinLiver And Spleen Dnase proteinLs-Dnase proteinLsd proteinDNASE1L3 proteinDHP2 proteinDNAS1L3 protein |
Information sourced from Uniprot.org