Human DNAM-1/CD226 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001648)
SPECIFICATIONS
HostHEK293
ConjugationBiotin
ImmunogenDNA sequence encoding Human DNAM-1/CD226 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
General Information
| Short Description | Recombinant-Human DNAM-1/CD226-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Biotin |
| Dilution Range | Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
| Formulation | Lyophilised from 0.2 ยตm filtered PBS solution pH7.4. |
| Storage Instruction | The lyophilized protein is stable for at least 1 year from date of receipt at-20ยฐC. |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ยตg (1EU/ยตg). |
Target Information
| Gene Symbol | CD226 |
| Gene ID | 10666 |
| Uniprot ID | CD226_HUMAN |
| Immunogen | DNA sequence encoding Human DNAM-1/CD226 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Immunogen Region | Glu19-Asn247 |
Additional Info
| Post Translational Modifications | PKC-mediated phosphorylation at Ser-329 is required for lipid raft recruitment. Phosphorylation of Tyr-322 requires association with lipid rafts and allows GRB2 binding and activation of subsequent signaling. |
| Function | Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells. Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells. Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes. |
| Protein Name | Cd226 AntigenDnax Accessory Molecule 1Dnam-1Cd Antigen Cd226 |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinLocalizes To Lipid Rafts |
| Alternative Protein Names | Cd226 Antigen proteinDnax Accessory Molecule 1 proteinDnam-1 proteinCd Antigen Cd226 proteinCD226 proteinDNAM1 protein |
Information sourced from Uniprot.org