• Biotinylated Human DNAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human DNAM-1 is greater than 95% as determined by SEC-HPLC.
  • Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human DNAM-1, His-Avi Tag with an affinity constant of 264nM as determined in SPR assay (Biacore T200).

Human DNAM-1/CD226 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001648)

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STJP001648

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human DNAM-1/CD226-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: CD226
Gene ID: 10666
Uniprot ID: CD226_HUMAN
Immunogen Region: Glu19-Asn247
Immunogen: DNA sequence encoding Human DNAM-1/CD226 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications PKC-mediated phosphorylation at Ser-329 is required for lipid raft recruitment. Phosphorylation of Tyr-322 requires association with lipid rafts and allows GRB2 binding and activation of subsequent signaling.
Function Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells. Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells. Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes.
Protein Name Cd226 Antigen
Dnax Accessory Molecule 1
Dnam-1
Cd Antigen Cd226
Database Links Reactome: R-HSA-198933
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Localizes To Lipid Rafts
Alternative Protein Names Cd226 Antigen protein
Dnax Accessory Molecule 1 protein
Dnam-1 protein
Cd Antigen Cd226 protein
CD226 protein
DNAM1 protein

Information sourced from Uniprot.org

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