| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | 1.Measured by its binding ability in a functional ELISA.Immobilized Human DKK-1 at 5 Mu g/mL (100 Mu L/well) can bind Human LRP-5 with a linear range of 0.027-0.559 Mu g/mL.|2.Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase prod |
| Determination Method: | <0.1 EU/Mu g |
| Gene Symbol: | DKK1 |
| Gene ID: | 22943 |
| Uniprot ID: | DKK1_HUMAN |
| Immunogen: | Recombinant Human Dkk-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr32-His266) of human DKK-1 (Accession #NP_036374.1) fused with 6×His tag at the C-terminus. |
| Immunogen Region: | Thr32-His266 |
| Immunogen Sequence: | TLNSVLNSNAIKNLPPPLGG AAGHPGSAVSAAPGILYPGG NKYQTIDNYQPYPCAEDEEC GTDEYCASPTRGGDAGVQIC LACRKRRKRCMRHAMCCPGN YCKNGICVSSDQNHFRGEIE ETITESFGNDHSTLDGYSRR TTLSSKMYHTKGQEGSVCLR SSDCASGLCCARHFWSKICK PVLKEGQVCTKHRRKGSHGL EIFQRCYCGEGLSCRIQKDH HQASNSSRLHTCQRH |
| Tissue Specificity | Placenta. |
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity. |
| Protein Name | Dickkopf-Related Protein 1Dickkopf-1Dkk-1Hdkk-1Sk |
| Database Links | Reactome: R-HSA-201681Reactome: R-HSA-3772470Reactome: R-HSA-5339717 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Dickkopf-Related Protein 1 proteinDickkopf-1 proteinDkk-1 proteinHdkk-1 proteinSk proteinDKK1 proteinUNQ492 proteinPRO1008 protein |
Information sourced from Uniprot.org
