Human Dihydrofolate Reductase/DHFR protein (Recombinant) (His-Tag) (STJP016513)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Dihydrofolate Reductase/DHFR-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M Nacl 2mM DTT, and 30% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Specific activity is > 2000pmol/min/ug, and is defined as the amount of enzyme that converts 1.0pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C. |
Target Information
| Gene Symbol | DHFR |
| Gene ID | 1719 |
| Uniprot ID | DYR_HUMAN |
| Accession Number | NP_000782.1 |
| Immunogen | Human |
| Immunogen Region | 1-187aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain. |
| Function | Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2. |
| Protein Name | Dihydrofolate Reductase |
| Database Links | Reactome: R-HSA-1474151Reactome: R-HSA-196757Reactome: R-HSA-69205 |
| Cellular Localisation | MitochondrionCytoplasm |
| Alternative Protein Names | Dihydrofolate Reductase proteinDHFR protein |
Information sourced from Uniprot.org