Human DC-SIGN/CD209 protein (Recombinant) (C-His) (STJP001484)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human DC-SIGN/CD209 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln59-Ala404) of human CD209/DC-SIGN (Accession #NP_066978.1) fused with a 6ΓΒHis tag at the C-terminus.
General Information
| Short Description | Recombinant-Human DC-SIGN/CD209-C-His protein was developed in hek293 cells using the region Gln59-Ala404. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20ΓΒ°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. When 5 x 104 cells/well are added to Recombinant Human DC SIGN/CD209 Protein coated plates (5 Mu g/mL with 100 Mu L/well) |
| Determination Method | <0.1 EU/Mu g |
Target Information
| Gene Symbol | CD209 |
| Gene ID | 30835 |
| Uniprot ID | CD209_HUMAN |
| Immunogen | Recombinant Human DC-SIGN/CD209 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln59-Ala404) of human CD209/DC-SIGN (Accession #NP_066978.1) fused with a 6ΓΒHis tag at the C-terminus. |
| Immunogen Region | Gln59-Ala404 |
| Immunogen Sequence | QVSKVPSSISQEQSRQDAIY QNLTQLKAAVGELSEKSKLQ EIYQELTQLKAAVGELPEKS KLQEIYQELTRLKAAVGELP EKSKLQEIYQELTWLKAAVG ELPEKSKMQEIYQELTRLKA AVGELPEKSKQQEIYQELTR LKAAVGELPEKSKQQEIYQE LTRLKAAVGELPEKSKQQEI YQELTQLKAAVERLCHPCPW EWTFFQGNCYFMSNSQRNWH DSITACKEVGAQLVVIKSA |
Additional Info
| Function | Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells. (Microbial infection) Acts as an attachment receptor for HIV-1 and HIV-2. (Microbial infection) Acts as an attachment receptor for Ebolavirus. (Microbial infection) Acts as an attachment receptor for Cytomegalovirus. (Microbial infection) Acts as an attachment receptor for HCV. (Microbial infection) Acts as an attachment receptor for Dengue virus. (Microbial infection) Acts as an attachment receptor for Measles virus. (Microbial infection) Acts as an attachment receptor for Herpes simplex virus 1. (Microbial infection) Acts as an attachment receptor for Influenzavirus A. (Microbial infection) Acts as an attachment receptor for SARS-CoV. (Microbial infection) Acts as an attachment receptor for Japanese encephalitis virus. (Microbial infection) Acts as an attachment receptor for Lassa virus. Acts as an attachment receptor for Marburg virusn. (Microbial infection) Acts as an attachment receptor for Respiratory syncytial virus. (Microbial infection) Acts as an attachment receptor for Rift valley fever virus and uukuniemi virus. (Microbial infection) Acts as an attachment receptor for West-nile virus. (Microbial infection) Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of bacterial pathogen antigens, including Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA. Recognition of M.tuberculosis by dendritic cells occurs partially via this molecule. |
| Protein Name | Cd209 AntigenC-Type Lectin Domain Family 4 Member LDendritic Cell-Specific Icam-3-Grabbing Non-Integrin 1Dc-SignDc-Sign1Cd Antigen Cd209 |
| Database Links | Reactome: R-HSA-5621575Reactome: R-HSA-8851680Reactome: R-HSA-9833110 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type Ii Membrane ProteinIsoform 2: Cell MembraneIsoform 3: Cell MembraneIsoform 4: Cell MembraneIsoform 5: Cell MembraneIsoform 6: SecretedIsoform 7: SecretedIsoform 8: SecretedIsoform 9: SecretedIsoform 10: SecretedIsoform 11: SecretedIsoform 12: Secreted |
| Alternative Protein Names | Cd209 Antigen proteinC-Type Lectin Domain Family 4 Member L proteinDendritic Cell-Specific Icam-3-Grabbing Non-Integrin 1 proteinDc-Sign proteinDc-Sign1 proteinCd Antigen Cd209 proteinCD209 proteinCLEC4L protein |
Information sourced from Uniprot.org