| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human DAG1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
DAG1 |
| Gene ID: |
1605 |
| Uniprot ID: |
DAG1_HUMAN |
| Specificity: |
This kit recognizes Human DAG1 in samples. No significant cross-reactivity or interference between Human DAG1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in a variety of fetal and adult tissues. In epidermal tissue, located to the basement membrane. Also expressed in keratinocytes and fibroblasts. |
| Post Translational Modifications | Alpha-dystroglycan: O-glycosylated. POMGNT1 catalyzes the initial addition of N-acetylglucosamine, giving rise to the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety and thus providing the necessary basis for the addition of further carbohydrate moieties. Heavily O-glycosylated comprising of up to two thirds of its mass and the carbohydrate composition differs depending on tissue type. Mucin-type O-glycosylation is important for ligand binding activity. O-mannosylation is found in high abundance in both brain and muscle where the most abundant glycan is Sia-alpha-2-3-Gal-beta-1-4-Glc-NAc-beta-1-2-Man. In muscle, glycosylation on Thr-317, Thr-319 and Thr-379 by a phosphorylated O-mannosyl glycan with the structure 2-(N-acetylamido)-2-deoxygalactosyl-beta-1,3-2-(N-acetylamido)-2-deoxyglucosyl-beta-1,4-6-phosphomannose is mediated by like-acetylglucosaminyltransferase (LARGE1) protein and is required for laminin binding. The O-glycosyl hexose on Thr-367, Thr-369, Thr-372, Thr-381 and Thr-388 is probably mannose. O-glycosylated in the N-terminal region with a core 1 or possibly core 8 glycan. The brain form displays a unique glycosylation pattern which is absent in other tissues.this form shows enhanced binding to laminin LAMA5 compared to the skeletal muscle form. Alpha-dystroglycan: (Microbial infection) O-mannosylation is required for binding lymphocytic choriomeningitis virus, Old World Lassa fever virus, and clade C New World arenaviruses. Beta-dystroglycan: N-glycosylated. Autolytic cleavage produces the alpha and beta subunits. In cutaneous cells, as well as in certain pathological conditions, shedding of beta-dystroglycan can occur releasing a peptide of about 30 kDa. SRC-mediated phosphorylation of the PPXY motif of the beta subunit recruits SH2 domain-containing proteins, but inhibits binding to WWW domain-containing proteins, DMD and UTRN. This phosphorylation also inhibits nuclear entry. |
| Function | The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Alpha-dystroglycan: Extracellular peripheral glycoprotein that acts as a receptor for extracellular matrix proteins containing laminin-G domains. Receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells. Also acts as a receptor for laminin LAMA5. Beta-dystroglycan: Transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity. Alpha-dystroglycan: (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus glycoprotein and class C new-world arenaviruses. Acts as a Schwann cell receptor for Mycobacterium leprae, the causative organism of leprosy, but only in the presence of the G-domain of LAMA2. |
| Protein Name | Dystroglycan 1DystroglycanDystrophin-Associated Glycoprotein 1 Cleaved Into - Alpha-DystroglycanAlpha-Dg - Beta-DystroglycanBeta-Dg |
| Database Links | Reactome: R-HSA-3000171Reactome: R-HSA-3000178Reactome: R-HSA-5083628Reactome: R-HSA-5083629Reactome: R-HSA-5083633Reactome: R-HSA-5173105Reactome: R-HSA-9010553Reactome: R-HSA-9619665 |
| Cellular Localisation | Alpha-Dystroglycan: SecretedExtracellular SpaceBeta-Dystroglycan: Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasmCytoskeletonNucleusNucleoplasmCell MembraneSarcolemmaPostsynaptic Cell MembraneThe Monomeric Form Translocates To The Nucleus Via The Action Of Importins And Depends On RanNuclear Transport Is Inhibited By Tyr-892 PhosphorylationIn Skeletal MuscleThis Phosphorylated Form Locates To A Vesicular Internal Membrane CompartmentIn Muscle CellsSarcolemma Localization Requires The Presence Of Ank2While Localization To Costameres Requires The Presence Of Ank3Localizes To Neuromuscular Junctions (Nmjs) In The Presence Of Ank2In Peripheral NervesLocalizes To The Schwann Cell MembraneColocalizes With Erm Proteins In Schwann-Cell Microvilli |
| Alternative CLIA Names | Dystroglycan 1 CLIA kitDystroglycan CLIA kitDystrophin-Associated Glycoprotein 1 Cleaved Into - Alpha-Dystroglycan CLIA kitAlpha-Dg - Beta-Dystroglycan CLIA kitBeta-Dg CLIA kitDAG1 CLIA kit |
| Specificity | This kit recognizes Human DAG1 in samples. No significant cross-reactivity or interference between Human DAG1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-105 | 98 |
| EDTA plasma(n=8) | 99-112 | 105 |
| Cell culture media(n=8) | 89-100 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 183.70 | 640.59 | 1981.45 | 185.71 | 579.16 | 1843.92 |
| Standard deviation | 15.16 | 65.79 | 173.18 | 23.12 | 62.03 | 120.96 |
| CV (%) | 8.25 | 10.27 | 8.74 | 12.45 | 10.71 | 6.56 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
