| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 1.0 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC. The IC50 value approximately is 26.3 nM. |
| Gene Symbol: | CST4 |
| Gene ID: | 1472 |
| Uniprot ID: | CYTS_HUMAN |
| Immunogen Region: | Ser21-Ala141 |
| Immunogen: | Recombinant Human Cystatin-S/CST4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser21-Ala141) of human Cystatin S (Accession #NP_001890.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | SSSKEENRIIPGGIYDADLN DEWVQRALHFAISEYNKATE DEYYRRPLQVLRAREQTFGG VNYFFDVEVGRTICTKSQPN LDTCAFHEQPELQKKQLCSF EIYEVPWEDRMSLVNSRCQE A |
| Post Translational Modifications | Phosphorylated at both its N- and C-terminal regions. |
| Function | This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. |
| Protein Name | Cystatin-SCystatin-4Cystatin-Sa-IiiSalivary Acidic Protein 1 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Cystatin-S proteinCystatin-4 proteinCystatin-Sa-Iii proteinSalivary Acidic Protein 1 proteinCST4 protein |
Information sourced from Uniprot.org